FSnet March 23/07

Depp girl had E. coli virus (bacteria -- dp)

Illegal cheese suspected in outbreak

Caterers drop supplier after listeria scare

Food inspectors serve closure

Spinach growers get aid provision as food-safety-standards bill stalls

Conagra prepares Peter Pan comeback

Health Hazard Alert - Marcella's Garden brand Olives may contain dangerous bacteria

County's recycled water is safe for crops

Effect of tannins on the in Vitro growth of Escherichia coli O157:H7 and in Vivo growth of generic Escherichia coli excreted from steers

Prevalence, numbers, and subtypes of Campylobacter jejuni and Campylobacter coli in uncooked retail meat samples

Sodium chloride enhances adherence and aggregation and strain variation influences invasiveness of Listeria monocytogenes strains

Evaluation of the transfer of Listeria monocytogenes from stainless steel and high-density polyethylene to bologna and American cheese

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Depp girl had E. coli virus (bacteria -- dp)
23.mar.07
The Sun (UK)
Thomas Whitaker
http://www.thesun.co.uk/article/0,,2-2007130572,00.html
Film hunk Johnny Depp’s daughter was seriously ill with the lethal E.coli virus, it was revealed last night.
The story says that production staff on the star’s new movie were told that the illness caused Lily-Rose’s kidneys to shut down — and she was lucky to survive.
A senior source on Depp’s new film Sweeney Todd was quoted as saying last night, "Everyone gasped when we were given the real reason for Lily-Rose’s illness. It was touch and go for her for a while."
Pirates of the Caribbean star Depp, 43, refused to continue filming until his daughter, seven, was out of danger. But after returning to the family home in France, Lily-Rose is said to be on the road to recovery.
Devoted dad Johnny — who also has son Jack, four, with partner Vanessa Paradis, 34 — kept a bedside vigil for his sick daughter in a London hospital earlier this month.



 

Illegal cheese suspected in outbreak
23.mar.07
Chicago Sun Times (Illinios)
Steve Lord
http://www.suntimes.com/news/metro/310257,CST-NWS-cheese23.article
Kane County authorities were cited as saying Thursday that illegally manufactured and distributed Mexican-style cheeses may be linked to a recent salmonella outbreak.
Kane County Health Department officials declined to name the cheeses as the sole source of the outbreak, but they issued a public warning about queso fresco or queso cotija cheese, adding that they found salmonella in dairy purchased at Aurora's El Paso Carniceria Chico.
The story says that at least 32 salmonella related illnesses were reported in Aurora, Elgin, Carpentersville and Dundee since January 2006. Most of those affected are Latino.
Kane County Health Department deputy director Paul Kuehnert recommended disposing any white Mexican cheese from area Mexican grocery stores.
Kuehnert said the unlicensed cheese manufactured in Illinois is made from unpasteurized milk and fails to meet many state- and county-mandated safeguards.



 

Caterers drop supplier after listeria scare
22.mar.07
Courier .co.uk (UK)
Stuart Woledge
http://www.thisiskentandeastsussex.co.uk/displayNode.jsp?nodeId=143201&command=displayContent&sourceNode=143126&contentPK=16928193&folderPk=82941&pNodeId=143133
Two Catering companies with outlets in the Sevenoaks area have dropped one of their suppliers following a listeria scare.
The disease was found in sandwiches supplied by Ashford-based Anchor Catering after it was detected during a routine test by Ashford Borough Council.
The Food Standards Agency (FSA) has warned Anchor's customers to be aware of a possible outbreak.
These include frozen food manufacturer Apetito in Dunton Green, which also distributes meals on wheels to the elderly, and U Select It, which runs a canteen in Moor Road, Sevenoaks.



 

Food inspectors serve closure
22.mar.07
Richmond Review (Virginia)
Martin van den Hemel
http://www.richmondreview.com/portals-code/list.cgi?paper=45&cat=23&id=858101&more=
A restaurant in Yaohan Centre’s food court became the first in the city to be closed down this year for health reasons following a routine inspection by Richmond Health Services.
The story says that Chun Hing Cuisine, at 1040-3700 No. 3 Rd., was shut down on March 8 when a city inspector noted a number of deficiencies with how food was being handled and stored as well as the restaurant’s handwashing facilities. The closure was lifted on Monday, March 12.
The report, available at www.foodinspectionweb.vcha.ca, was quoted as saying, "An uncovered bucket of vegetables was found storing on top of another uncovered bucket with partially cooked pork."
The inspection revealed that pork chops were thawing in standing water, rather than under running water, as required.
The restaurant was also using cardboards for floor mats, shelf liners and grease absorbers.
Restaurants are inspected once per year by public health inspectors, with more frequent inspections for establishments with a history of poor food handling or other deficiencies. Inspections are conducted without prior warning.
Inspectors look for unsafe cooking practices, obtaining food from unapproved sources, poor employee hygiene, infestations, inadequate dish washing/sanitizing, inadequate refrigeration.
Reports on restaurants in Richmond and the Vancouver Coastal Health Authority region are available on-line at www.foodinspectionweb.vcha.ca. A list of restaurant closures is available at www.vch.ca/environmental/food.



 

Spinach growers get aid provision as food-safety-standards bill stalls
21.mar.07
USA Today
Ken Dilanian
http://www.usatoday.com/news/washington/2007-03-21-spinach-aid_N.htm?csp=34
WASHINGTON -- Darryl Howard's mom, Betty, was among those who died after eating contaminated spinach last fall at her home in Washington state, he says.
He was stunned to learn last week that the emergency bill to fund the Iraq war and Hurricane Katrina relief included $25 million to compensate spinach growers hurt when consumers stopped buying their products.
Backers of the spinach provision say it is designed to help innocent growers whose businesses took a hit even though their greens weren't contaminated. The insertion into an emergency war funding bill of $3.7 billion to benefit spinach growers, peanut farmers and others in agribusiness underscores a Washington truism: Some interests are more special than others.
The story says that agribusiness spent $84 million lobbying Congress and contributed $44.1 million to federal campaigns in 2005 and 2006, according to public records tallied by the Center for Responsive Politics, a non-partisan organization that tracks spending by those seeking to influence federal policy.
While the spinach aid provision was placed in a must-pass spending bill that has been scheduled for a vote Friday in the House, legislation to toughen food safety standards is stalled. A bill to create an independent food safety agency, introduced in February by Rep. Rosa DeLauro, D-Conn., is pending in the Energy and Commerce, and Agriculture Committees, where similar DeLauro proposals have died for years.
The story says tha proponents say some level of subsidies are crucial to preserving domestic food production. Michael Doyle who directs the University of Georgia's Center for Food Safety, was quoted as saying, "If we want farmers to grow crops in the U.S., we're going to have to subsidize them."



 

Conagra prepares Peter Pan comeback
23.mar.07
TMC Net (Nebraska)
http://www.tmcnet.com/usubmit/2007/03/23/2438690.htm
(Omaha World-Herald (NE) -- Chief Executive Gary Rodkin was cited as saying Thursday that ConAgra Foods' Peter Pan peanut butter will remain off store shelves at least through late May as the company prepares for the brand's return after a salmonella-linked recall.
Rodkin was further cited as saying while announcing ConAgra's third-quarter earnings that company officials are preparing a marketing plan to reintroduce the product, adding, "I am convinced that this event will make us a stronger and better company."
Peanut butter generates about $150 million in annual sales for ConAgra, but the company will have to forgo revenue from that segment at least through its fourth quarter this year.



 

Health Hazard Alert - Marcella's Garden brand Olives may contain dangerous bacteria
22.mar.07
Canadian Food Inspection Agency
OTTAWA -- The Canadian Food Inspection Agency (CFIA) and Excelsior Foods Inc. are warning the public not to consume Marcella's Garden brand Olives described below because these products may be contaminated with Clostridium botulinum. Toxins produced by this bacteria may cause botulism, a life-threatening illness.
Only the following Marcella's Garden brand Olives sold in 600 ml plastic tubs bearing a best before date of 31/07/2007 are affected by this alert.
1. Baresana Olives, lot 009/6, UPC 0 62136 90067 8.
2. Calabrese Olives, lot 012/6, UPC 0 62136 90070 8.
3. Cerignola Olives, lot 011/6, UPC 0 62136 90069 2.
4. Jumbo Stuffed Olives, lot 011/9, UPC 0 62136 90071 5.
5. Kalamata Olives, lot 005/6, UPC 0 62136 90066 1.
6. Nocellara Olives, lot 010/6, UPC 0 62136 90068 5.
These olives, imported from Italy, were distributed in British Columbia, Alberta, Manitoba and Ontario.
There have been no reported illnesses associated with the consumption of these olives.
Food contaminated with Clostridium botulinum toxin may not look or smell spoiled. Consumption of food contaminated with the toxin may cause nausea, vomiting, fatigue, dizziness, headache, double vision, dry throat, respiratory failure and paralysis. In severe cases of illness, people may die.
The importer, Excelsior Foods Inc., Toronto, Ontario, is voluntarily recalling the affected products from the marketplace. The CFIA is monitoring the effectiveness of the recall.
For more information, consumers and industry may call one of the following numbers:
Excelsior Foods Inc., Daniel Violante, at 1-800-845-5636.
CFIA at 1 800 442-2342. 8:00 a.m. to 4:00 p.m. local time, Monday to Friday.
For information on Clostridium botulinum, visit the Food Facts web page at
http://www.inspection.gc.ca/english/fssa/concen/cause/botulisme.shtml
For information on receiving recalls by e-mail, or for other food safety facts, visit our web site at www.inspection.gc.ca.



 

County's recycled water is safe for crops
22.mar.047
The Californian
Keith Israel
http://www.californianonline.com/apps/pbcs.dll/article?AID=/20070322/OPINION/703220327/1014
Keith Israel, general manager of the Monterey Regional Water Pollution Control Agency, writes that James Smallwood's Feb. 28 Soapbox, "Recycled water is suspect," merits a response. He questions the use of recycled water for irrigation of crops in parts of northern Monterey County. As a matter of fact, the recycled water used does meet the highest standards of the California Water Recycling Criteria also known as Title 22.
These criteria require that the tertiary water be disinfected such that the produced water contains less than 2.2 most probable number (MPN) of total coliform bacteria per 100 mL. Recycled water produced by MRWPCA does meet these criteria as shown in daily monitoring conducted over the Past nine years.
Also during this time, we have supplied over 30 billion gallons of safe recycled water to area growers.
Scientific studies over the past several decades have shown that the California standard is indicative of pathogen-free water as safe as, or safer than, other water sources used for irrigation of food crops. Numerous virus assays in Monterey and other tertiary treatment plants in California have shown that viruses are consistently deactivated through the tertiary treatment and disinfection processes. Smallwood also wonders about the possibility if the "cyst-forming bacteria" would "wake up" in real world environmental conditions. He is probably referring to the re-growth phenomenon commonly observed with distribution of potable water as well as recycled water. While bacterial re-growth can occur in pipelines that have lower levels of water disinfection or where the water is allowed to stagnant, MRWPCA maintains a disinfection residual in the distribution system to prevent regrowth from happening. Since the pathogenic form of E. coli has not been present in any of the samples of influent in the treatment plant there is no reason for it to re-grow or "wake up" if it was not there in the first place. Plus, the extensive plant disinfection process would deactivate any pathogens prior to release of the final product.
In summary, the regional water recycled project provides water that has been proven safe for irrigation for crops and other uses.
Additionally, the Monterey County Environmental Health Department confirms the safety of our recycled water with its own testing and can provide more information. Call 755-4507.



 

Effect of tannins on the in Vitro growth of Escherichia coli O157:H7 and in Vivo growth of generic Escherichia coli excreted from steers
01.mar.07
Journal of Food Protection, Volume 70, Number 3
Min, Byeng R.1; Pinchak, William E.1; Anderson, Robin C.2; Callaway, Todd R.2
Abstract:
The effect of commercially available chestnut and mimosa tannins in vitro (experiment 1) or in vivo (experiment 2) on the growth or recovery of Escherichia coli O157:H7 or generic fecal E. coli was evaluated. In experiment 1, the mean growth rate of E. coli O157:H7, determined via the measurement of optical density at 600 nm during anaerobic culture in tryptic soy broth at 37°C, was reduced (P < 0.05) with as little as 400 É g of either tannin extract per ml of culture fluid. The addition of 200, 400, 600, 800, and 1,200 É g of tannins per ml significantly (P < 0.01) reduced the specific bacterial growth rate when compared with the nontannin control. The specific growth rate decreased with increasing dose levels up to 800 É g of tannins per ml. Bacterial growth inhibition effects in chestnut tannins were less pronounced than in mimosa tannins. Chestnut tannin extract addition ranged from 0 to 1,200 É g/ml, and a linear effect (P < 0.05) was observed in cultures incubated for 6 h against the recovery of viable cells, determined via the plating of each strain onto MacConkey agar, of E. coli O157:H7 strains 933 and 86-24, but not against strain 6058. Similar tests with mimosa tannin extract showed a linear effect (P < 0.05) against the recovery of E. coli O157:H7 strain 933 only. The bactericidal effect observed in cultures incubated for 24 h with the tannin preparations was similar, although it was less than that observed from cultures incubated for 6 h. When chestnut tannins (15 g of tannins per day) were infused intraruminally to steers fed a Bermuda grass hay diet in experiment 2, fecal E. coli shedding was lower on days 3 (P < 0.03), 12 (P = 0.08), and 15 (P < 0.001) when compared with animals that were fed a similar diet without tannin supplementation. It was concluded that dietary levels and sources of tannins potentially reduce the shedding of E. coli from the gastrointestinal tract.
Affiliations: 1: Texas Agricultural Experiment Station, P.O. Box 1658, Vernon, Texas 76385, USA 2: U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, 2881 F & B Road, College Station, Texas 77845, USA



 

Prevalence, numbers, and subtypes of Campylobacter jejuni and Campylobacter coli in uncooked retail meat samples
01.mar.07
Journal of Food Protection, Volume 70, Number 3
Wong, Teck Lok1; Hollis, Lauren1; Cornelius, Angela1; Nicol, Carolyn2; Cook, Roger3; Hudson, John Andrew1
A national quantitative survey of Campylobacter jejuni and Campylobacter coli in 1,011 uncooked retail meat samples (beef, unweaned veal, chicken, lamb and mutton, and pork) was undertaken from August 2003 to June 2004 to establish baseline proportionality data. The presence, number, and type of Campylobacter present in each sample was assessed. Prevalences of C. jejuni and C. coli were 89.1% in chicken, 9.1% in pork, 6.9% in lamb and mutton, 3.5% in beef, and 10% in unweaned veal. C. jejuni was identified in the majority of positive samples (246 of 259). In chicken samples positive for C. jejuni, 40.2% had counts of <0.3 most probable number (MPN)/g, 50.5% had 0.3 to 10.0 MPN/g, 8.8% had 10.1 to 50.0 MPN/g, and 0.5% had 110 MPN/g. In other meats (49 samples), Campylobacter counts were ?0.3 MPN/g, except for one unweaned veal sample at >10.9 MPN/g. Penner serotyping and SmaI macrorestriction genotyping using pulsed-field gel electrophoresis with 247 isolates revealed 17 Penner serotypes and 56 electrophoresis profiles. Seven Penner serotypes (HS1 complex, 2, 4 complex, 6, 11, 27, and 42) were represented by 10 or more isolates from chicken. When data from both typing methods were combined, 62 sero-genotypes were generated. In a comparison of these sero-genotypes with historical data for isolates from human cases, 71% of the beef isolates, 50% of the lamb and mutton isolates, 50% of the pork isolates, 41% of the chicken isolates, and 25% of the unweaned veal isolates were common to both sources. These results provide baseline proportionality profiles of Campylobacter in these five meats and will facilitate exposure assessment in combination with other information such as consumption data and subsequent quantitative risk assessment
Affiliations: 1: Food Safety Programme, Christchurch Science Centre, Institute of Environmental Science and Research, P.O. Box 29-181, Christchurch, New Zealand 2: Enteric Reference Laboratory, Kenepuru Science Centre, Institute of Environmental Science and Research, P.O. Box 50-348, Porirua, New Zealand 3: New Zealand Food Safety Authority, P.O. Box 2835, Wellington, New Zealand



 

Sodium chloride enhances adherence and aggregation and strain variation influences invasiveness of Listeria monocytogenes strains
01.mar.07
Journal of Food Protection, Volume 70, Number 3
Jensen, Anne1; Larsen, Marianne H.2; Ingmer, Hanne2; Vogel, Birte Fonnesbech1; Gram, Lone1
Abstract:
Some subtypes of Listeria monocytogenes can persist in the food-processing industry, but the reasons for such persistence are not known. In the present study, 10 strains of L. monocytogenes representing known persistent randomly amplified polymorphic DNA (RAPD) types from fish processing plants were compared to eight strains of different RAPD type and origin (clinical, food, and animal). All 18 strains of L. monocytogenes had similar growth patterns at different temperatures (5 or 37°C) or different salinities (0.5 or 5% NaCl), and all strains formed a thin layer of adhered cells on a plastic surface when cultured in tryptone soya broth (TSB) with a total of 1% glucose. Many ready-to-eat foods, such as cold-smoked fish, contain NaCl at concentrations of 2 to 5%, and NaCl is present in the processing environment. Adding NaCl to TSB changed the adhesion patterns of all strains, and all adhered better when NaCl was added. Also, the addition of NaCl caused a marked aggregation of 13 of the strains; however, 5 of the 18 strains did not aggregate in the presence of up to 5% NaCl. The aggregates stuck to the plastic surface, and this occurred in all but one of the persistent RAPD types. Four strains represented one particular RAPD type that has been isolated as a persistent RAPD type in several fish processing plants for up to 10 years. Because this RAPD type often can contaminate fish products, it is important to address its potential virulence. The 18 strains differed markedly in their ability to invade Caco-2 cells, and the four strains representing the universal persistent RAPD type were the least invasive (102 to 103 CFU/ml), whereas other strains invaded Caco-2 cells at levels of 104 to 105 CFU/ml. Five of the 18 strains belonged to the genetic lineage 1 and were the most invasive. Although the most commonly isolated persistent RAPD type was low invasive, it is important to understand why moderate salinity facilitates aggregation and biofilm formation, for this understanding can be beneficial in developing procedures to reduce processing plant contamination.
Affiliations: 1: Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, DTU bldg. 221, DK-2800 Kgs. Lyngby, Denmark 2: Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, Stigbøjlen 8, DK-1870 Frederiksberg C, Denmark



 

Evaluation of the transfer of Listeria monocytogenes from stainless steel and high-density polyethylene to bologna and American cheese
01.mar.07
Journal of Food Protection, Volume 70, Number 3
Rodríguez, Andrés1; McLandsborough, Lynne A.1
Abstract:
The objective of this study was to determine the factors involved in the transfer of Listeria monocytogenes from surfaces to foods. We evaluated the influence of surface type (stainless steel and high-density polyethylene), inoculation method (biofilm growth and attached cells), hydration level (visibly dry and wet), and food type (bologna and American cheese). Each experiment included all 16 combinations and was repeated 11 times. A four-strain cocktail of L. monocytogenes was used to inoculate stainless steel and high-density polyethylene either as growing biofilms or attached cells. Slides were placed on a universal testing machine and brought into contact with food at a constant pressure (45 kPa) and time (30 s). Food slices were blended, the number of transferred cells was determined by plating, and the efficiency of transfer (EOT) was calculated. The results strongly suggest that stainless steel surfaces transferred more L. monocytogenes to foods than did polyethylene (P = 0.05). Independent of the surface, biofilms tended to transfer more L. monocytogenes to foods (EOT = 0.57) than did attached cells (EOT = 0.16). Among foods, L. monocytogenes was transferred to bologna more easily than to cheese (P < 0.05). The impact of hydration on transfer was significantly higher for dried biofilms growing on stainless steel (P < 0.05). No significant differences for hydration were seen under other conditions (P > 0.05). We hypothesize that drying weakens cell-to-cell interactions in biofilms and cell-to-surface interactions of biofilms and thus allows increased transfer of cells to food products.
Document Type: Research article
Affiliations: 1: Department of Food Science, University of Massachusetts, Chenoweth Laboratory, Amherst, Massaschusetts 01003, USA




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