FSnet Feb. 23/05 -- II

EU says 11 states may be hit by UK food dye scare

Canadian Cattlemen's Association represents interests of Cattle Producers in R-CALF Lawsuit

Perspective: Are we the best corporate citizens that we can be? MeatNews editor Dr. Dom Castaldo asks processors and suppliers

From the NMA: Meat Group editorial director Steve Bjerklie says the message from the NMA convention is that meat marketing must be all about the customer

Genome of deadly amoeba shows surprising complexity

Computational fluid dynamics analysis for process impact assessment during thermal pasteurization of intact eggs

Extended study on the influence of z-value(s) of single and multicomponent time-temperature integrators on the accuracy of quantitative thermal process assessment

Combined effects of coating, modified atmosphere packaging, and gamma irradiation on quality maintenance of ready-to-use carrots (Daucus carota)

Occurrence of mycotoxin in farro samples from Southern Italy

Inactivation of pathogens inoculated into prepared seafood products for manufacturing kimbab, steamed rice rolled in dried seaweed, by gamma irradiation

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EU says 11 states may be hit by UK food dye scare
February 23, 2005
Reuters
BRUSSELS - The European Commission was cited as saying on Wednesday that 11 European Union states may have imported tainted food from Britain containing an illegal dye that could lead to an increased risk of cancer.
Britain has taken 427 soups, sauces and ready-meals off supermarket shelves since Feb. 18 when the banned dye Sudan 1 was found in a batch of chilli powder used by British tea and pickle maker Premier Foods Plc to make Worcester sauce.
The sauce was added to a range of food products.
The food scare has hit Belgium, Cyprus, Denmark, France, Greece, Ireland, Italy, Malta, the Netherlands, Portugal and Spain, said European Commission spokesman Philip Tod.



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Canadian Cattlemen's Association represents interests of Cattle Producers in R-CALF Lawsuit
February 23, 2005
From a press release
CALGARY - The Canadian Cattlemen's Association (CCA), on behalf of Canadian cattle producers, has filed for permission to provide information in the R-CALF vs. United States Department of Agriculture law suit.
Legal counsel representing CCA and Canadian cattle producers has filed an amicus curiae ("friend of the court") brief in the case being heard in the United States District Court in Billings, Montana. Amicus briefs are legal documents filed by non-litigants in court cases which include additional information or arguments that those outside parties wish to have considered in that particular case. The CCA brief focuses on the economics of re-opening the border to both live cattle and an expanded list of beef products, and argues that re-opening the border will not harm the U.S. beef industry. An amicus brief filed by the Government of Canada focuses on the scientific arguments for re-opening the border.
"The purpose of filing the amicus brief is to ensure that the judge hearing the case has all the relevant information needed to make an informed decision," says Dennis Laycraft, Executive Vice President of the CCA. "The information being filed dispels the myth that there are vast numbers of cattle waiting to cross the border on March 7. It also points out that consumer confidence has remained high in both Canada and the U.S. and re-opening the border will not negatively impact that confidence." The judge hearing the case will decide if the brief filed by CCA, and amicus briefs filed by other interested parties, will be accepted. The CCA brief is available for viewing on the CCA website www.cattle.ca. CCA is the National Voice for the Beef Cattle Industry, representing over 90,000 Cattle Producers.



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Perspective: Are we the best corporate citizens that we can be? MeatNews editor Dr. Dom Castaldo asks processors and suppliers
February 23, 2005
MeatNews.com Volume 7, Issue 9
Dr. Dom Castaldo
http://www.meatnews.com/index.cfm?fuseaction=Article&artNum=9081
When I was growing up in New Jersey, the only connection I had to the meat processing industry was the local butchers that ran stores in the Italian urban neighborhood where my father grew up and where most of my aunts, uncles, and cousins still lived. Mom would always get sausage and ground pork and veal from those little shops that dotted the neighborhood. Every butcher shop – they also did duty as delicatessens that sold hard-to-pronounce Italian meats, bakeries, seafood shops, and convenience stores – had their own signature meats, particularly sausage. They usually made it “in the back.” The big problem was consistency. A butcher may make a delicious batch of sausage this week; but next week, don’t count on it being the same.
The one thing that these long-gone butchers were consistent about was that they played an active role in the neighborhood. They were good corporate citizens. Most of the time, they lived above their stores. If you needed something after closing, they would come down and get it for you. They sponsored neighborhood soccer and baseball teams – sometimes they sponsored more than one team. Either in the front window or behind the counter, they would display the trophies that their teams won. When the church had its summer bazaar, they got the church supplies at cost. The butchers of my youth were loyal to the neighborhood, and the neighborhood was loyal to them – for the most part.
The demise of the local butcher shop is simple. Most of the children of the old neighborhood’s residents moved away to the suburbs – or further away -- and bought their meat in supermarkets. Also, the butcher’s kids didn’t want to be butchers. So the shops closed. Some are now two-story homes; others are video stores or they sell something else. The butchers now work in processing plants. I don’t even know who cuts my steaks or grinds my sausage.
This gets me to thinking: Do the big meat companies – the new butcher shops – have the same sense of being good corporate citizens as the neighborhood butchers?
I think so.
A number of years ago, I was talking with the public relations director of a large poultry company. The company’s plant was located in a residential area (The plant was there before the houses.) I asked how the company was prepared for an accident. The PR director said an incident had already occurred. She told me that during the previous summer, the plant’s refrigeration system failed. She said the company immediately called a meeting with the local residents, explained the situation, and told them that although they were working to remove the meat and fix the refrigeration system, things were likely to smell. The company wasn’t wrong. But, the residents understood and didn’t complain or file any lawsuits. The processor acted like a good neighbor.
In December when the tsunami struck Asia, meat companies rallied to do what they do best – provide high quality food to the victims. During Christmas holidays, I hear about meat processors providing turkeys, hams, and other products to homeless shelters and the like to feed the hungry. Most of the meat plants I’ve visited participate in the United Way Drive.
I’ve been reading a lot about Tyson’s corporate neighborliness lately. When the company had to stop processing beef at several of its plants, the company still paid the laid-off workers a major portion of their wages. Without Tyson’s generosity, many of those workers wouldn’t have made it financially. They may have lost their houses or cars or had to relocate. The towns were they lived would have suffered too. Last week, the company expanded its educational reimbursement program. I am certain that other processors are being equally generous to their employees and their communities.
We as an industry – meat processors and suppliers – must maintain – and even improve -- our excellent track record as corporate citizens.



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From the NMA: Meat Group editorial director Steve Bjerklie says the message from the NMA convention is that meat marketing must be all about the customer
February 23, 2005
MeatNews.com Volume 7, Issue 9
http://www.meatnews.com/index.cfm?fuseaction=Article&artNum=9080
According to Ken Parnell, who guided Wal-Mart’s meat, seafood, and delicatessen divisions for 17 years, “Everything should be based on the customer. We must be agents for the customer.”
Speaking at a roundtable session devoted to new meat marketing ideas, part of the program at the annual National Meat Association convention being held this week in Las Vegas, Nevada, Parnell, who was a meat buyer for Safeway prior to his career at Wal-Mart, and who founded Parnell Leaton Consulting last year, commented that Wal-Mart “changed some things in the industry that we thought should be changed,” and he described how Wal-Mart’s case-ready program for fresh meat gave the retailing giant extraordinary control over inventory management. But, he noted, the emphasis must always be on the customer. “Shelf-life belongs to the customer,” he said. “Even animal welfare belongs to the customer. That’s why you do it.”
Steve Sands of Premium Protein Products, a Nebraska meat packer and processor aiming at niche markets, said his company is focused specifically “on the consumer’s desire for branded product.” He commented: “We are about maximizing carcass utilization by focusing on value-added products sold to a complimentary set of customers.” The firm operates a slaughter plant in Hastings, Nebraska, and a processing plant in Lincoln. “Our driver is our value-added operation,” he said. Premium Protein Products manages several branded programs, including brands that cover organic and other production protocols. “One of our goals is eliminating discounts for the producer,” he noted.
Gary Pfeiffer of Superior Farms, the Washington state-headquartered lamb processor, said his company has found opportunity by focusing on “a high-quality eating experience for consumers.” As part of that effort, Superior, which had been committed exclusively to U.S.-grown lamb, recently began importing Australian lamb to round out the company’s product line.
Also paying attention to a specific niche, Ernie Phinney of Western Grasslands Beef, a California meat company exclusively raising grass-fed livestock, called grass-fed “a new category for a new era.” He emphasized: “This is not your grandfather’s grass-fed beef. This is your great-great-great grandfather’s grass-fed beef. He admitted that in the United States grass-fed product still fights a market conception that it’s not as tender or flavorful as grain-fed beef, which came to dominate beef production for retail beef in this country in the post-war years. But, he commented: “There are millions of unhappy consumers out there who don’t think the beef they’ve been buying in the supermarket over the last several years tastes very good.”
John Nalivka of Sterling Marketing and Meat Processing magazine’s contributing editor for economics (He assembles the annual “Meat Facts” report for i>Meat Processing publisher, Watt Publishing Co.), commented that with regard to source-verification programs and animal identification, which increasingly are being used for marketing as well as control, “What we’re really talking about is managing risk. We’re talking about trying to reduce volatility and increase predictability. And those are the things that an effective marketing program has to have.” He said effective traceback offers the opportunity to “match a live animal to a specific consumer market.”
Ed Lopes of Villa Roma Sausage, a southern California processor of retail processed products, commented that newspaper advertising and in-store taste-demonstrating, both traditional ways of marketing food products, especially specialties like sausage, remain effective tools. Villa Roma, he said, has developed a sophisticated method for determining the true cost and sales benefits of specific advertisements and in-store demonstrations.
Steve Bjerklie, editorial director of Watt Publishing Co.’s Meat Group, concluded the roundtable by noting that “the organic companies as well as some others with creative, innovative marketing programs have really shown the rest of the industry how much power there is in marketing narratives. If you can sell the consumer the story and context of your product rather than just offering an item with an attractive price, your margins can improve significantly, as these companies have proven,” he said.



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Genome of deadly amoeba shows surprising complexity
February 23, 2005
The Institute for Genomic Research (TIGR)
The genome sequence of the parasitic amoeba Entamoeba histolytica, a leading cause of severe diarrheal disease in developing countries, includes an unexpectedly complex repertoire of sensory genes as well as a variety of bacterial-like genes that contribute to the organism’s unique biology.
The report, which appears in the February 24 issue of Nature, presents the first genome-wide study of an amoeba. It is also the first genome sequence to be published from this class of amitochondrial human pathogens.
The analysis reveals the degradation of the E. histolytica genome in its transition from a free-living organism into a parasite of the human gut. At the same time, scientists also cataloged the retention and expansion of some gene families characteristic of more complex organisms.
Detailing the first systematic study of relatively recent horizontal gene transfer into a protist, scientists report evidence in the DNA sequence that E. histolytica likely picked up a significant number of its metabolic genes from bacterial co-inhabitants of the human gut. Identification of these genes sheds new light on the unusual shared biology between the parasitic amoeba and anaerobic gut bacteria.
The E. histolytica genome sequence is expected to help in the development of new vaccines as well as diagnostic tests that can distinguish the amoeba's most deadly strains. The parasite infects an estimated 50 million people and causes as many as 100,000 deaths a year -- second only to malaria as a cause of morbidity and mortality from a protist. The disease caused by E. histolytica is called amebiasis.
The sequencing of E. histolytica was a collaborative effort led by The Institute for Genomic Research (TIGR) in Rockville, MD, and by the Wellcome Trust Sanger Institute in the U.K. The project was supported by grants from the National Institute of Allergy and Infectious Diseases (NIAID), which is part of the National Institutes of Health, and from the Wellcome Trust.
The study’s first author, TIGR scientist Brendan Loftus, says the contents of the amoeba’s genome surprised many scientists. “The parts list we identified implies that E. histolytica may have re-engineered aspects of its metabolism,” says Loftus, citing the protist’s loss of some genes and its apparent gain of other bacterial-like genes through lateral transfer. “This study will provoke interest in what secrets may lie undiscovered in the sequences of other supposedly ‘simple’ amoeba genomes.”
Named for its effectiveness in killing other cells, E. histolytica was long considered to be a ‘primitive’ organism – originating from the time that bacterial lineages diverged from eukaryotic lineages – because the amoeba lacked many of the visible traits of eukaryotic cells (such as endoplasmic reticulum, golgi apparatus and mitochondria) and also shared some bacteria-like traits.
While the Nature study catalogs a reduced genome (sometimes characteristic of an organism’s transition to a parasitic lifestyle), Loftus says scientists found ample evidence of a variety of gene families characteristic of more complex organisms. For example, a family of membrane receptors appears to be one of the mechanisms by which the parasite senses the presence of its human host and translates environmental cues into signaling events, which are processed by the parasite.
Another unusual feature of the genome is the presence of an unprecedented number of tRNA (transfer RNA) genes, which constitute nearly 10 percent of the sequence data collected for the project. These genes appear to be present within the genome in long arrays whose structural or functional significance is not yet known.
“Clearly, this amoeba has genes that allow it to sense certain facets of its environment and respond to those cues,” says Neil Hall, a TIGR scientist who is the senior author of the Nature study. He did most of his work on the E. histolytica genome while in his previous position at Sanger.
Matthew Berriman of Sanger said, "The results give a fascinating glimpse of how this ancient parasite evolved and highlight unusual metabolic processes that may be exploitable as drug targets."
The project also identified some large families of surface proteins that may help mediate the amoeba's ability to evade the human immune system. This could explain why the parasite can stay hidden in the body for years at a time. Such information may help researchers find better means to harness the immune system to eradicate infection through vaccine development.
A number of the metabolic adaptations and stratagems identified from the E. histolytica genome also have commonalities with those reported in two other amitochondrial human pathogens of global importance: Trichomonas vaginalis and Giardia lamblia.
“The enzymes that form the basis of these shared metabolic strategies are substantially different from human enzymes, making them potential targets of inhibitors that could form the basis of new drug therapies," says TIGR President Claire M. Fraser, who supervised the Institute’s role in the project.
E. histolytica is a voracious predator of bacteria and shares a close relationship in the human gut with its bacterial neighbors. Because the parasite uses the same methods to kill bacteria as it uses to damage human cells and cause disease, the level of certain bacteria in the colon can be an important determinant of the amoeba’s virulence.
Biomedical scientists say the E. histolytica sequence will help researchers develop new tools to predict which of the millions of people who ingest amoeba cysts in contaminated fluids will actually develop the disease.
“Every aspect of research is changed by having the genome sequenced,” says Dr. William A. Petri, Jr., a collaborator who is chief of the University of Virginia Health System’s Division of Infectious Diseases. He says the DNA typing from the genome sequenced is allowing the development of “new diagnostic tests that help distinguish the most deadly substrains of the parasite.”
Dr. Alok Bhattacharya, a collaborator at the School of Life Sciences at India’s Jawaharlal Nehru University, says having the genome sequence “will help to identify new drug and vaccine targets” and also may help researchers understand why the disease hits only a small fraction of those who are hosts to the parasite. “Intestinal infections are one of the major health problems in developing countries and the number of people who are infected with amoeba cysts is enormous,” he says.
Typically, those cysts are transmitted when people ingest contaminated food or water. The disease can cause liver damage but more often causes dysentery, a severe diarrhea that is often associated with blood in the feces. Unchecked, the diarrhea associated with the disease can be fatal, especially in children. One field study in Bangladesh found that E. histolytica infection occurred at least once in 80 percent of 300 children. Over a period of four years, about a third of the children suffered from amebic colitis, an ulceration of the stomach lining.
Petri says the genome data will help researchers find more about what makes some people innately resistant to infection by E. histolytica, and what acquired immune responses can protect people from re-infection. Says Petri: "The genome project has spawned rapid discovery of the parasite’s mechanisms for replication, gene expression, motility, metabolism, and the killing of host cells.”
The Institute for Genomic Research (TIGR) is a not-for-profit research institute based in Rockville, Maryland. TIGR, which sequenced the first complete genome of a free-living organism, has been at the forefront of the genomic revolution since the institute was founded in 1992. The Institute’s scientists conduct research involving the structural, functional, and comparative analysis of genomes and gene products in viruses, bacteria, archaea, and eukaryotes.



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Computational fluid dynamics analysis for process impact assessment during thermal pasteurization of intact eggs
February 2005
Journal of Food Protection Volume 68, Number 2, p. 366-374
Siegfried Denys ; Jan G. Pieters ; Koen Dewettinck
http://www.foodprotection.org/QuickLinks.htm
Abstract: Transient temperature and albumen velocity profiles during thermal pasteurization of intact eggs were studied using a commercial computational fluid dynamics (CFD) package. Simulated temperature profiles were in close agreement with experimental data for eggs of different sizes. Convective heat transfer only occurred in the egg white fraction, and conductive heat transfer only occurred in the yolk. For process assessment, a generally accepted kinetic inactivation model for Salmonella Enteritidis was incorporated into the CFD analysis. Minimum process times and temperatures needed to provide equivalent pasteurization at 5-log reductions of the target microorganism were obtained on a theoretical basis. The combination of CFD analysis and inactivation kinetics can be very useful for assessing pasteurization of intact eggs and can enable processors to gain a better understanding of these processes and to establish process conditions for consumer-safe eggs.



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Extended study on the influence of z-value(s) of single and multicomponent time-temperature integrators on the accuracy of quantitative thermal process assessment
February 2005
Journal of Food Protection Volume 68, Number 2, p. 384-395
Yann P. Guiavarch ; Ann M. van Loey ; Marc E. Hendrickx
http://www.foodprotection.org/QuickLinks.htm
Abstract
The possibilities and limitations of single- and multicomponent time-temperature integrators (TTIs) for evaluating the impact of thermal processes on a target food attribute with a ztarget value different from the zTTI value(s) of the TTI is far from sufficiently documented. In this study, several thousand time-temperature profiles were generated by heat transfer simulations based on a wide range of product and process thermal parameters and considering a ztarget value of 10°C and a reference temperature of 121.1°C, both currently used to assess the safety of food sterilization processes. These simulations included 15 different ztarget-10°CF121.1°C values in the range 3 to 60 min. The integration of the time-temperature profiles with zTTI values of 5.5 to 20.5°C in steps of 1°C allowed generation of a large database containing for each combination of product and process parameters the correction factor to apply to the process value FmultiTTI, which was derived from a single- or multicomponent TTI, to obtain the target process value 10°CF121.1°C. The table and the graph results clearly demonstrated that multicomponent TTIs with z-values close to 10°C can be used as an extremely efficient approach when a single-component TTI with a z-value of 10°C is not available. In particular, a two-component TTI with z1 and z2 values respectively above and below the ztarget value (10°C in this study) would be the best option for the development of a TTI to assess the safety of sterilized foods. Whatever process and product parameters are used, such a TTI allows proper evaluation of the process value10°CF121.1°C.



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Combined effects of coating, modified atmosphere packaging, and gamma irradiation on quality maintenance of ready-to-use carrots (Daucus carota)
February 2005
Journal of Food Protection Volume 68, Number 2, p. 353-359
R. Lafortune ; S. Caillet ; M. Lacroix
http://www.foodprotection.org/QuickLinks.htm
Abstract
The objective of this study was to evaluate the effect of an edible coating combined with modified atmosphere (MA; 60% O2, 30% CO2, and 10% N2) packaging and gamma irradiation on the microbiological stability and physicochemical quality of minimally processed carrots. A coating based on calcium caseinate and whey protein isolates was used. Coated and uncoated peeled minicarrots were packed under the MA or air (78.1% N2, 20.9% O2, and 0.036% CO2), irradiated at 0.5 or 1 kGy, and stored at 4 ± 1°C for 21 days. Samples were evaluated periodically for aerobic plates counts (APCs) and physicochemical properties (firmness, white discoloration, and whiteness index). Gamma irradiation did not significantly affect the physicochemical properties of the carrots (P > 0.05). Microbiological analysis revealed that for uncoated carrots irradiation at 0.5 and 1 kGy under air and MA reduced the APCs by 3.5 and 4 log CFU/g and by 4 and 4.5 log CFU/g, respectively. For coated carrots, irradiation at 0.5 and 1 kGy under air and MA reduced the APCs by 4 and 4.5 log CFU/g and by 3 and 4.25 log CFU/g, respectively. The coating was able to protect carrots against dehydration during storage under air. Coating and irradiation at 1 kGy were also able to protect carrot firmness during storage under air. MA packaging retarded whitening of uncoated carrots but had a detrimental effect on firmness. The edible coating used in this study did not significantly inhibit (P > 0.05) microbial growth on carrots.



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Occurrence of mycotoxin in farro samples from Southern Italy
February 2005
Journal of Food Protection Volume 68, Number 2, p. 416-420
Raffaello Castoria ; Giuseppe Lima ; Rosalia Ferracane ; Andalberto Ritieni
http://www.foodprotection.org/QuickLinks.htm
Abstract
The occurrence of nine mycotoxins and of contamination by pre- and postharvest fungal pathogens of cereals was investigated in samples of stored Triticum monococcum L., Triticum dicoccon Schrank (emmer), and Triticum spelta L. (spelt). In Italy, all three species are collectively referred to as farro. The samples examined were harvested in summer 2000 from eight different sites in southern Italy. Conventional fluorimetric and diode array-based high-performance liquid chromatography (HPLC) analyses and HPLC-mass spectrometry analyses were used to identify fumonisin B1 in five samples (up to 70.00 g/ kg), ochratoxin A in seven samples (up to 4.07g/kg), and beauvericin in three samples (up to 4.44 mg/kg). Enniatin B was detected in one sample (30.00 g/kg), but no zearalenone or fusaproliferin was found. Deoxynivalenol and aflatoxins were not evaluated. The potentially mycotoxigenic fungal species detected were Alternaria alternata, Fusarium proliferatum, Fusarium tricinctum, Penicillium verrucosum, and Penicillium chrysogenum. This is the first report of the natural occurrence of mycotoxins in farro samples.



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Inactivation of pathogens inoculated into prepared seafood products for manufacturing kimbab, steamed rice rolled in dried seaweed, by gamma irradiation
February 2005
Journal of Food Protection Volume 68, Number 2, p. 396-402
Cheorun Jo ; Na Young Lee ; Ho Jin Kang ; Sang Pil Hong ; Young Ho Kim ; Jae Kyung Kim ; Myung Woo Byun
http://www.foodprotection.org/QuickLinks.htm
Abstract
Three prepared seafood products for manufacturing a laver (dried seaweed) roll, a traditional and rapidly growing ready-to-eat meal in Korea, were selected and the effects of irradiation treatment for eliminating pathogens of public health significance were investigated. The pathogens tested were Salmonella Typhimurium, Escherichia coli, Staphylococcus aureus, and Listeria ivanovii. The radiation sensitivity (D10-values or the dose required to inactivate 90% of a population) of these organisms ranged from 0.23 to 0.62 kGy in imitation crab leg, 0.31 to 0.44 kGy in surimi gel, and 0.27 to 0.44 kGy in dried seaweed. The growth of all four test organisms inoculated (108 CFU/g) into these foods was inhibited by irradiation during 24 h of postirradiation storage regardless of the temperature (10, 20, and 30°C). L. ivanovii was not detected after a 3-kGy treatment, but the other pathogens were not detected following irradiation at 2 kGy. These studies indicated that low-dose irradiation (2 kGy or less) of prepared seafood materials can keep them microbiologically safe before manufacturing a readyto-eat prepared meal, a laver roll.
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